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- Title
CD3<sup>bright</sup> signals on γδ T cells identify IL-17A-producing Vγ6Vδ1<sup>+</sup> T cells.
- Authors
Paget, C; Chow, M T; Gherardin, N A; Beavis, P A; Uldrich, A P; Duret, H; Hassane, M; Souza‐Fonseca‐Guimaraes, F; Mogilenko, D A; Staumont‐Sallé, D; Escalante, N K; Hill, G R; Neeson, P; Ritchie, D S; Dombrowicz, D; Mallevaey, T; Trottein, F; Belz, G T; Godfrey, D I; Smyth, M J
- Abstract
Interleukin-17A (IL-17A) is a pro-inflammatory cytokine that has an important role at mucosal sites in a wide range of immune responses including infection, allergy and auto-immunity. γδ T cells are recognized as IL-17 producers, but based on the level of CD3 expression, we now define the remarkable ability of a CD3bright γδ T-cell subset with an effector memory phenotype to rapidly produce IL-17A, but not interferon-γ. CD3bright γδ T cells uniformly express the canonical germline encoded Vγ6/Vδ1+ T-cell receptor. They are widely distributed with a preferential representation in the lungs and skin are negatively impacted in the absence of retinoic acid receptor-related orphan receptor gammat expression or endogenous flora. This population responded rapidly to various stimuli in a mechanism involving IL-23 and NOD-like receptor family, pyrin domain containing 3 (NLRP3)-inflammasome-dependent IL-1β. Finally, we demonstrated that IL-17-producing CD3bright γδ T cells responded promptly and strongly to pneumococcal infection and during skin inflammation. Here, we propose a new way to specifically analyze IL-17-producing Vγ6/Vδ1+ T cells based on the level of CD3 signals. Using this gating strategy, our data reinforce the crucial role of this γδ T-cell subset in respiratory and skin disorders.
- Subjects
INTERLEUKIN-17; T cells; CYTOKINES; ESCHERICHIA coli; STAPHYLOCOCCUS aureus; PHENOTYPES
- Publication
Immunology & Cell Biology, 2015, Vol 93, Issue 2, p198
- ISSN
0818-9641
- Publication type
Article
- DOI
10.1038/icb.2014.94