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- Title
Development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Flavobacterium psychrophilum.
- Authors
Fujiwara-Nagata, E.; Eguchi, M.
- Abstract
Flavobacterium psychrophilum is the causative agent of bacterial cold-water disease and rainbow trout fry syndrome of salmonids. The pathogen has been reported from all regions in the world involved in salmonid aquaculture, but also from natural fresh-water environments. We established a quantitative loop-mediated isothermal amplification of DNA (LAMP) method to estimate quantities of F. psychrophilum. LAMP primers were designed based on the sequence of the DNA topoisomerase IV subunit B gene, parE, of F. psychrophilum. parE LAMP exhibited a high specificity for the parE gene of F. psychrophilum but not for other related species. parE LAMP detected the gene in a wide range of concentrations from 2.0 × 101 to 2.0 × 109 copies/reaction within 70 min and revealed a good correlation between threshold times and gene copy number.
- Subjects
BACTERIAL kidney disease (Fish disease); SALMONIDAE infections; SALMON farming; RAINBOW trout; DNA; GENES
- Publication
Journal of Fish Diseases, 2009, Vol 32, Issue 10, p873
- ISSN
0140-7775
- Publication type
Article
- DOI
10.1111/j.1365-2761.2009.01066.x