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- Title
Sulfur-Doped Organosilica Nanodots as a Universal Sensor for Ultrafast Live/Dead Cell Discrimination.
- Authors
Li, Yan-Hong; Zeng, Jia; Wang, Zihao; Wang, Tian-Yu; Wu, Shun-Yu; Zhu, Xiao-Yu; Zhang, Xinping; Shan, Bai-Hui; Gao, Cheng-Zhe; Wang, Shi-Hao; Wu, Fu-Gen
- Abstract
Rapid and accurate differentiation between live and dead cells is highly desirable for the evaluation of cell viability. Here, we report the application of the orange-emitting sulfur-doped organosilica nanodots (S-OSiNDs) for ultrafast (30 s), ultrasensitive (1 μg/mL), and universal staining of the dead bacterial, fungal, and mammalian cells but not the live ones, which satisfies the requirements of a fluorescent probe that can specifically stain the dead cells. We further verify that the fluorescence distribution range of S-OSiNDs (which are distributed in cytoplasm and nucleus) is much larger than that of the commercial dead/fixed cell/tissue staining dye RedDot2 (which is distributed in the nucleus) in terms of dead mammalian cell staining, indicating that S-OSiNDs possess a better staining effect of dead cells than RedDot2. Overall, S-OSiNDs can be used as a robust fluorescent probe for ultrafast and accurate discrimination between dead and live cells at a single cell level, which may find a variety of applications in the biomedical field.
- Subjects
FLUORESCENT probes; STAINS &; staining (Microscopy); CYTOPLASM; CELL survival; DETECTORS
- Publication
Biosensors (2079-6374), 2022, Vol 12, Issue 11, p1000
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios12111000