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- Title
Kanatlılardan Escherichia coli O157 İzolasyonu Üzerine Çalışmalar.
- Authors
ABAY, Seçil; AYDIN, Fuat; ERTAŞ, Nurhan; HIZLISOY, Harun; ERDOĞDU, Sevgi; GÖNÜLALAN, Zafer
- Abstract
In this study, the determination of Escherichia coli O157 prevalence in different poultry originated materials was aimed. For this purpose, 400 meat samples and 400 intestinal contents of Broilers grown in Kayseri, 400 stool samples of laying hens and 80 swap samples taken from various poultry processing units (boiling tank water, walls and removing unit of intestinal organs) were evaluated in this study. The materials were taken between March 2010 and February 2011 with three month intervals (each season). The isolation period was formed in phases including pre-enrichment of E.coli broth with novobiocin (20mg/L), immunomagnetic separation, and seeding on SMAC (MacConkey with sorbitol) and CHROM agar. Considering the colonies appeared on SMAC and CHROM agar, 116 of 1280 samples were found to be E.coli O157 positive. The 334 colonies taken from 116 samples were investigated with phenotypic tests for E.coli and all of them were confirmed as E.coli. These isolates were subjected to latex agglutination test for E.coli O157 and in total 8 samples, 2 broiler stools and 6 poultry processing samples, were found positive. These 8 suspicious E.coli O157 strains were found E.coli O157 negative by Polymerase Chain Reaction. The results showed that poultry and their poultry meat samples collected in this study did not carry E.coli O157 in Kayseri and the investigation of greater number of poultry originated samples from different locations for E.coli O157 is suggested.
- Subjects
ESCHERICHIA coli; POULTRY; BROILER chickens; NOVOBIOCIN; IMMUNOMAGNETIC separation; AGGLUTINATION tests; POLYMERASE chain reaction
- Publication
Journal of Faculty of Veterinary Medicine, Erciyes University / Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 2014, Vol 11, Issue 1, p1
- ISSN
1304-7280
- Publication type
Article