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- Title
High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants.
- Authors
Fan, Tingting; Cheng, Yanhao; Wu, Yuechao; Liu, Shishi; Tang, Xu; He, Yao; Liao, Shanyue; Zheng, Xuelian; Zhang, Tao; Qi, Yiping; Zhang, Yong
- Abstract
Cytosine base editors (CBEs) and adenine base editors (ABEs) enable precise C-to-T and A-to-G edits. Recently, ABE8e, derived from TadA-8e, enhances A-to-G edits in mammalian cells and plants. Interestingly, TadA-8e can also be evolved to confer C-to-T editing. This study compares engineered CBEs derived from TadA-8e in rice and tomato cells, identifying TadCBEa, TadCBEd, and TadCBEd_V106W as efficient CBEs with high purity and a narrow editing window. A dual base editor, TadDE, promotes simultaneous C-to-T and A-to-G editing. Multiplexed base editing with TadCBEa and TadDE is demonstrated in transgenic rice, with no off-target effects detected by whole genome and transcriptome sequencing, indicating high specificity. Finally, two crop engineering applications using TadDE are shown: introducing herbicide resistance alleles in OsALS and creating synonymous mutations in OsSPL14 to resist OsMIR156-mediated degradation. Together, this study presents TadA-8e derived CBEs and a dual base editor as valuable additions to the plant editing toolbox. Base editors are a powerful but underexplored tool for genetic engineering and directed evolution of plants. Here, the authors investigate the editing efficiency and specificity of TadA-8e-derived cytosine base editors and dual base editor TadDE in rice and tomato.
- Subjects
GENOME editing; CYTOSINE; WHOLE genome sequencing; HERBICIDE resistance; GENETIC engineering; TRANSGENIC rice
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-49473-w