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- Title
Chemomechanical coupling of F<sub>1</sub>-ATPase under hydrolysis conditions.
- Authors
Rikiya Watanabe; Hiroyuki Noji
- Abstract
F1-ATPase (F1) is the smallest rotary motor protein that couples ATP hydrolysis/synthesis to rotary motion in a highly reversible manner. F1 is unique compared with other motor proteins because of its high efficiency and reversibility in converting chemical energy into mechanical work. To determine the energy conversion mechanism of F1-ATPase, we developed a novel single-molecule manipulation technique with magnetic tweezers and determined the timing of Pi release, which was the last unknown piece of the chemomechanical coupling scheme of F1. The established fundamental chemomechanical coupling scheme provides evidence to explain the high reversibility between catalysis and mechanical work.
- Subjects
ADENOSINE triphosphatase; HYDROLYSIS; CHEMICAL energy conversion; CATALYSIS; CHEMICAL reactions; SINGLE molecules
- Publication
Biophysics (13492942), 2012, Vol 8, p73
- ISSN
1349-2942
- Publication type
Article
- DOI
10.2142/biophysics.8.73