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- Title
The extensin protein SAE1 plays a role in leaf senescence and is targeted by the ubiquitin ligase SINA4 in tomato.
- Authors
Lu, Han; Niu, Xiangli; Fan, Youhong; Yuan, Yulin; Huang, Li; Zhao, Bingyu; Liu, Yongsheng; Xiao, Fangming
- Abstract
Extensins are hydroxyproline-rich glycoproteins and generally play a structural role in cell wall integrity. In this study, we determined a novel role of tomato (Solanum lycopersicum) SENESCENCE-ASSOCIATED EXTENSIN1 (SAE1) in leaf senescence. Both gain- and loss-of-function analyses suggest that SAE1 plays a positive role in leaf senescence in tomato. Transgenic plants overexpressing SAE1 (SAE1 -OX) exhibited premature leaf senescence and enhanced dark-induced senescence, whereas SAE1 knockout (SAE1 -KO) plants displayed delayed development-dependent and dark-induced leaf senescence. Heterologous overexpression of SlSAE1 in Arabidopsis also led to premature leaf senescence and enhanced dark-induced senescence. In addition, the SAE1 protein was found to interact with the tomato ubiquitin ligase SlSINA4, and SlSINA4 promoted SAE1 degradation in a ligase-dependent manner when co-expressed in Nicotiana benthamiana leaves, suggesting that SlSINA4 controls SAE1 protein levels via the ubiquitin–proteasome pathway. Introduction of an SlSINA4 -overexpression construct into the SAE1 -OX tomato plants consistently completely eliminated accumulation of the SAE1 protein and suppressed the phenotypes conferred by overexpression of SAE1. Taken together, our results suggest that the tomato extensin SAE1 plays a positive role in leaf senescence and is regulated by the ubiquitin ligase SINA4.
- Subjects
UBIQUITIN; UBIQUITIN ligases; NICOTIANA benthamiana; TRANSGENIC plants; TOMATOES; PROTEINS; GLYCOPROTEINS
- Publication
Journal of Experimental Botany, 2023, Vol 74, Issue 18, p5635
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erad242