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- Title
Duodenum histometry in experimental diabetes-induced rats and effects of melatonin.
- Authors
E. D., İpek; H., Başaloğlu
- Abstract
Objective: Hyperglycemia causes damage to all tissues, organs due to production of excess reactive oxygen species. Whole gastrointestinal tract is affected by diabetes, from the oral cavity to the esophagus, from the stomach to the small-large intestines. We aimed to investigate powerful antioxidant melatonin effects on the duodenum. Methods: 16 weeks old male Wistar rats were used. Each group consists of eight animal; control group (K), diabetesgroup (D), melatonin group (M) and melatonin-treated-diabetes-group (DM). Diabetes induce 60 mg/kg/i.p. streptozotocin injection in D and DM. Subsequently, 10mg/kg/i.p./day melatonin was administered to DM and M for 6 weeks. Weight change, blood glucose levels were recorded weekly. At the end of work, duodenum samples were embedded in paraffin and 5 pm sections were stained with hematoxylin&eosin. The lengthwidth of the villi, crypt depth, duodenum, artery-vein diameters, tunica muscularis thickness were measured on 10 sections with AnalySIS LS Starter program. Differences between groups in parametric data were evaluated with OneWay ANOVA, non-parametric data with Kruskal-Wallis test. Results: Blood glucose levels were higher in the D and DM, whereas the change in body weight was low (p<0.05). Villus length, tunica muscularis thickness and goblet cell count were lower in D (p<0.05). Crypta depth was higher in D than M. Arterial diameter was not different between the groups, whereas the diameter of the vein was higher in the D and DM, and the diameter of the duodenum was higher in the D (p<0.05). Conclusion: Melatonin have positive effects on duodenal morphology, its combination with blood glucose homeostasis agents in diabetics may be effective against oxidative stress.
- Subjects
DUODENUM; BLOOD sugar; KRUSKAL-Wallis Test; EPITHELIAL cells; RATS
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue Supplement2, pS192
- ISSN
1307-8798
- Publication type
Article