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- Title
Investigation of neurotoxicity, neuropotectivity and neuroregenerative efficacy of Ginkgo biloba extract in vitro.
- Authors
Karavelioğlu, Zeynep; Koç, Rabia Çakır
- Abstract
Objective: Products of Ginkgo biloba (Gb) have been used for many years in a lot of countries especially in China as a traditional medicine for treatment of various conditions such as memory and concentration problems, depression, anxiety, dizziness and tinnitus due to the effects of many components such as kaempherol, ishorhamnetin, quercetin, ginkgolides and bilobalides. However, studies on the determination of neuroprotective and neuroregenerative effects of Gb extract at cellular level are quite limited. Therefore, the aim of this study is in vitro examination of neuroprotective and neuroregenerative effects of Gb extract on glioblastoma (U87) cells and neuroblastoma (SHSY-5Y) cells which are used as cell model in the treatment of diseases such as Alzheimer and Parkinson's due to their similarity to dopaminergic neurons. Methods: While U87 and SHSY-5Y cells were used for the investigation of neuroprotective and neuroregenerative effects of Gb extract, it's toxic effect was investigated on the mouse fibroblast cells (L929). Cells were cultured in DMEM F12 containing 10% FBS and 0.5% Pen-Strep. XTT test was performed for measuring cell viability and changes in the cell nucleus were observed by DAPI staining. Afterwards, Scratch Assay was applied to examine the regenerative effect of Gb extract on the cells. Results: According to the results, it was observed that Gb liquid extract did not show any toxic effect on the cell lines. In addition, it was observed that the extract increased the viability of SHSY- 5Y and U87 cell lines and showed neuroprotective effect at concentrations up to 50 mg/mL. Conclusion: In this study, the effective concentrations and neuroprotective and neuroregenerative effects of Gb extract on the cell lines were demonstrated in in vitro. According to the results, different formulations based on the Gb extract can be developed in the future. Special thanks to the IMMUNAT company for supplying the active substance.
- Subjects
CHINA; DOPAMINERGIC neurons; GINKGO; CHINESE medicine; CELL nuclei; NEUROTOXICOLOGY; PARKINSON'S disease; EXTRACTS
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2019, Vol 13, Issue S1, pS71
- ISSN
1307-8798
- Publication type
Article