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- Title
Improving rice grain length through updating the <italic>GS3</italic> locus of an elite variety Kongyu 131.
- Authors
Nan, Jianzong; Feng, Xiaomin; Wang, Chen; Zhang, Xiaohui; Wang, Rongsheng; Liu, Jiaxin; Yuan, Qingbo; Jiang, Guoqiang; Lin, Shaoyang
- Abstract
Background: Traditional crop breeding has made significant achievement meet food needs worldwide. However, the way has some inevitable problems including time-consuming, laborious, low predictability and reproducibility. In this study, we updated the <italic>GRAIN SIZE 3 (GS3)</italic> locus to improve the grain length of a major cultivate variety of Kongyu 131 at Heilongjiang Province, the northernmost region of China. High-resolution melting (HRM) analysis is used for single nucleotide polymorphism (SNP) genotyping.Results: The improved line introgressed about 117 kb segment including <italic>gs3</italic> allele from donor GKBR by using five SNP markers designed within and without <italic>GS3</italic> locus, and the background recovery ratio of the recurrent parent genome is about 99.55% that are detected by 219 SNP markers evenly distributed on the 12 chromosomes. The field trial indicates that grain length, 100-grain weight and total grain weight per plant of the improved line raised by 12.05%, 16.30% and 4.47%, respectively, compared with Kongyu 131.Conclusions: This result demonstrates that update the <italic>GS3</italic> locus is a feasible and efficient and accurate way can be applied to improve grain size of rice.
- Subjects
RICE breeding; PLANT genomes; SINGLE nucleotide polymorphisms; PLANT breeding; MELTING
- Publication
Rice (19398425), 2018, Vol 11, Issue 1, p1
- ISSN
1939-8425
- Publication type
Article
- DOI
10.1186/s12284-018-0217-2