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- Title
Molecular discrimination and identification of Acetobacter genus based on the partial heat shock protein 60 gene ( hsp60) sequences.
- Authors
Huang, Chien‐Hsun; Chang, Mu‐Tzu; Huang, Lina; Chua, Wen‐Shen
- Abstract
BACKGROUND To identify the Acetobacter species using phenotypic and genotypic ( 16S rDNA sequence analysis) technique alone is inaccurate. The aim of this study was to use the hsp60 gene as a target for species discrimination in the genus Acetobacter, as well as to develop species-specific polymerase chain reaction and mini-sequencing methods for species identification and differentiation. RESULTS The average sequence similarity for the hsp60 gene (89.8%) among type strains was significantly less than that for the 16S rRNA gene (98.0%), and the most Acetobacter species could be clearly distinguished. In addition, a pair of species-specific primer was designed and used to specifically identify Acetobacter aceti, Acetobacter estunensis and Acetobacter oeni, but none of the other Acetobacter strains. Afterwards, two specific single-nucleotide polymorphism primers were designed and used to direct differentiate the strains belonging to the species A. aceti by mini-sequencing assay. CONCLUSION The phylogenetic relationships in the Acetobacter genus can be resolved by using hsp60 gene sequencing, and the species of A. aceti can be differentiated using novel species-specific PCR combined with the mini-sequencing technology. © 2013 Society of Chemical Industry
- Subjects
ACETOBACTER; HEAT shock proteins; NUCLEOTIDE sequence; RECOMBINANT DNA; SINGLE nucleotide polymorphisms; POLYMERASE chain reaction
- Publication
Journal of the Science of Food & Agriculture, 2014, Vol 94, Issue 2, p213
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.6231