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- Title
Au@Zr-based metal–organic framework composite as an immunosensing platform for determination of hepatitis B virus surface antigen.
- Authors
Bajpai, Vivek K.; Haldorai, Yuvraj; Khan, Imran; Sonwal, Sonam; Singh, Mahendra Pal; Yadav, Seema; Paray, Bilal Ahamad; Jan, Basit Latief; Kang, Sung-Min; Huh, Yun Suk; Han, Young-Kyu; Shukla, Shruti
- Abstract
An ultrasensitive electrochemical immunosensor has been prepared using an immunofunctionalized zirconium (Zr)-based metal–organic framework (MOF) with gold (Au) decoration Au@UiO-66(NH2) composite-coated glassy carbon electrode (GCE) for the determination of infectious hepatitis B surface antigen (HBsAg). We fabricated GCE with specific composite via immune-functionalization using anti-HBsAg with Au nanoparticles embedded in UiO-66(NH2). The electrochemical sensing performance of the immunofunctionalized Au@UiO-66(NH2)/GCE with HBsAg was characterized by cyclic voltammetry and differential pulse voltammetry. Under optimized conditions, there was a linear dynamic relationship in the buffer system between the electrical signal and HBsAg levels over the range 1.13 fg mL−1–100 ng mL−1 (R2 = 0.999) with a detection limit of 1.13 fg mL−1. The total analysis time was 15 min per sample. Further validations were performed with HBsAg-spiked human serum samples, and similar detection limits as in the buffer system were observed with reduced signal intensities at lower concentrations of HBsAg (1, 10, and 100 fg mL−1) and minimal interference. The HBsAg electrochemical immunosensing assay had good selectivity and excellent reproducibility, thereby indicating its significant potential in the super-fast diagnosis of hepatitis B.
- Subjects
HEPATITIS associated antigen; METAL-organic frameworks; HEPATITIS B virus; CARBON electrodes; GOLD nanoparticles
- Publication
Microchimica Acta, 2021, Vol 188, Issue 11, p1
- ISSN
0026-3672
- Publication type
Article
- DOI
10.1007/s00604-021-05022-6