We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of the p53–tristetraprolin–stathmin-1 pathway on trophoblasts at maternal–fetal interface.
- Authors
Ma, Xiao-Ling; Li, Xiao-Cui; Tian, Fu-Ju; Zhang, Si-Ming; Liu, Xiao-Rui; Zhang, Yan; Fan, Jian-Xia; Lin, Yi
- Abstract
Problem: To reveal the effect of p53–tristetraprolin–stathmin-1 signaling on trophoblasts and recurrent spontaneous abortion (RSA). Method of study: Stathmin-1 (STMN1), p53, and tristetraprolin (TTP) expression in paraffin-embedded villus tissue was determined using immunohistochemistry. HTR-8/SVneo cells were treated with doxorubicin to activate p53; STMN1 and TTP levels were detected by quantitative reverse transcription–PCR and western blotting. Western blotting and immunofluorescence were used to investigate STMN1 expression after TTP overexpression or knockdown in HTR-8 cells. Results: STMN1 was downregulated and p53 was upregulated in the villus tissue from patients with RSA. Doxorubicin decreased STMN1 expression but enhanced TTP expression in HTR-8 cells. In vitro, TTP overexpression inhibited STMN1 production; TTP knockdown promoted it. TTP downregulated STMN1 expression in trophoblasts by directly binding its 3ʹ untranslated region. Conclusions: TTP modulates trophoblast function and interacts with STMN1 and p53, and is related to pregnancy outcomes.
- Subjects
P53 antioncogene; TRISTETRAPROLIN; STATHMIN; TROPHOBLAST; IMMUNOHISTOCHEMISTRY; POLYMERASE chain reaction; DEVELOPMENTAL biology; IMMUNOFLUORESCENCE
- Publication
PLoS ONE, 2017, Vol 12, Issue 6, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0179852