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- Title
The effect of microtubule stabilizer pretreatment in IVM program.
- Authors
Fesahat F.; Khalili M. A.; Khoradmehr A.; Anbari F.; Bazrgar M.; Taheri F.
- Abstract
Introduction: It has been reported that in vitro maturation (IVM) increases the risk of abnormal spindles and chromosome configurations of oocytes compared with oocytes matured in vivo. This is one possible explanation for the reduced developmental potential of IVM oocytes compared with those matured in vivo. Paclitaxel is known to stabilize the microtubules that constitute the spindle. The aim was to investigate whether pretreatment by a microtubule stabilizer, paclitaxel, would improve IVF outcomes in IVM program. Materials and Methods: In this ongoing experimental study, 75 GV oocytes were retrieved from 5 mice primed with rec-FSH for 48 hr. The immature oocytes were cultured 24-48 hr for IVM. After identifying mature oocytes by the presence of a first polar body extrusion under a stereomicroscope, Normal morphologically Mil oocytes were divided in control and 5 experiment groups. Experiment groups were incubated in presence of lpM paclitaxel for different times (30 min/group 1,1 hr/group 2,2 hr/group 3,3 hr/group 4,4 hr/ group 5 and group 6 as control) prior to IVF. Standard IVF program was performed for the 60 Mil oocytes after pretreatment with paclitaxel and embryo development was followed until blastocyst formation. Results: Total maturation rate of GV oocytes was 80% and fertilization rates of Mil oocytes were 85% in groups 6, 83% in groups 1, 2 and 3 and 74% and 65% in groups 4, 5 respectively. Blastocyst formation rates of embryos were 95% in group 1, 2 and 6, 60% in group 3 and 40% in groups 4 and 5. The final results, which include probable relationship between paclitaxel pretreatment and the aforementioned variables, will be presented. Conclusion: The preliminary results showed by performing critical concentration with optimized timing of microtubule stabilization procedure IVM, Mil oocytes could efficiently develop to the blastocyst stage. Paclitaxel may cause cell toxicity with irreversible harm on normal fertilization and embryo development in higher concentrations.
- Subjects
MICROTUBULES; PACLITAXEL; LABORATORY mice; DISSECTING microscopes; EXPERIMENTAL groups
- Publication
Iranian Journal of Reproductive Medicine, 2015, p35
- ISSN
1680-6433
- Publication type
Article