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- Title
Predicting value of high magnification sperm morphology evaluation in ICSI outcome.
- Authors
Ghazali, Sh.; Talebi, A. R.; Khalili, M. A.; Soleimani, M.; Razi, M. H.; Halvaei, I.; Nabi, A.
- Abstract
Introduction: Sperm quality can impact on early embryo development and pregnancy rate in intracytoplasmic sperm injection (ICSI) cycles, and sperm morphology as one of the principle parameters for predicting ICSI outcome has been addressed. To date, sperm morphology evaluation techniques have markedly improved, specially with introducing high magnification microscopy. The aim of this study was to assess value of high magnification sperm morphology evaluation in predicting ICSI outcome. Materials and Methods: This prospective study was conducted on sperm samples from 117 patients who attended our center. After sperm processing by density-gradient technique, prepared spermatozoa were stained by Giemsa, and their nuclear vacuoles, shape and size were then analyzed at 6000× magnification and classified into three classes according to their quality. Class I: spermatozoa having normal shape with no vacuoles, class II: spermatozoa having normal shape with vacuoles, class III: spermatozoa having abnormal shape with vacuoles. The rates of cleavage and pregnancy were assessed between different classes. The relationship between sperm morphology and progressive motility was also evaluated. Results: The percentage of class I was significantly higher in positive pregnancy cases than the negatives (13.0% vs. 11.9%, respectively, p=0 .01).No significant differences were obtained with respect to all sperm morphological classes with sperm progressive motility and cleavage rates. Conclusion: It appears that sperm nuclear normalcy positively affects pregnancy rate in ICSI cycles. Therefore, assessment of sperm morphological characteristics using Giemsa staining at high magnification can be applied as a predictive tool in ICSI program.
- Subjects
SPERMATOZOA analysis; RADIOGRAPHIC magnification; HEALTH outcome assessment; EMBRYOLOGY; PREGNANCY complications
- Publication
Iranian Journal of Reproductive Medicine, 2013, p70
- ISSN
1680-6433
- Publication type
Article