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- Title
The Spore Coat Protein CotE Facilitates Host Colonization by Clostridium difficile.
- Authors
Hong, Huynh A.; Ferreira, William T.; Hosseini, Siamand; Anwar, Saba; Hitri, Krisztina; Wilkinson, Anthony J.; Vahjen, Wilfried; Zentek, Jürgen; Soloviev, Mikhail; Cutting, Simon M.
- Abstract
Clostridium difficile infection (CDI) is an important hospital-acquired infection resulting from the germination of spores in the intestine as a consequence of antibiotic-mediated dysbiosis of the gut microbiota. Key to this is CotE, a protein displayed on the spore surface and carrying 2 functional elements, an N-terminal peroxiredoxin and a C-terminal chitinase domain. Using isogenic mutants, we show in vitro and ex vivo that CotE enables binding of spores to mucus by direct interaction with mucin and contributes to its degradation. In animal models of CDI, we show that when CotE is absent, both colonization and virulence were markedly reduced. We demonstrate here that the attachment of spores to the intestine is essential in the development of CDI. Spores are usually regarded as biochemically dormant, but our findings demonstrate that rather than being simply agents of transmission and dissemination, spores directly contribute to the establishment and promotion of disease.
- Subjects
CLOSTRIDIOIDES difficile; BACTERIAL spore germination; PEROXIREDOXINS; GUT microbiome; CHITINASE; BACTERIAL colonies
- Publication
Journal of Infectious Diseases, 2017, Vol 216, Issue 11, p1452
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jix488