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- Title
Circular RNA SMARCA5 Modulates Epithelial-Mesenchymal Transformation, Proliferation, and Metastasis of Nasopharyngeal Carcinoma Cells via microRNA-582-3p/Phosphatase and Tensin Homolog Axis.
- Authors
Wang, Hui; Ren, HaiTang
- Abstract
The action mechanism in which circular RNA (circ) SMARCA5 targeted nasopharyngeal carcinoma (NPC) cell proliferation, migration, invasion, and apoptosis via microRNA (miR)-582-3p/phosphatase and tensin homolog (PTEN) axis was explored. The examination was performed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), discovering that circSMARCA5 was elevated while miR-582-3p was silenced in NPC tissues and cells. E-cadherin and N-cadherin were detected. The results illustrated transfection with si-circSMARCA5 or miR-582-3p-mimic was available to repress cancer cell advancement, and E-cadherin was augmented. Transfection with pcDNA 3.1-circSMARCA5 or miR-582-3p-inhibitor was available to accelerate cancer cell advancement, and N-cadherin was augmented. MiR-582-3p-inhibitor blocked the suppression of si-circSMARCA5 on NPC. The si-PTEN blocked the malignant behavior of pcDNA 3.1-circSMARCA5 against NPC. The binding sites between circSMARCA5 and miR-582-3p and between miR-582-3p and PTEN were verified. Linear analysis results illuminated the expression pattern of circSMARCA5 was opposite to miR-582-3p, while the expression pattern of circSMARCA5 was positively associated with PTEN. In brief, the results of the research clarified circSMARCA5 modulated NPC cells' vital movement via the miR-582-3p/PTEN molecular axis.
- Subjects
CIRCULAR RNA; NASOPHARYNX cancer; REVERSE transcriptase polymerase chain reaction; DNA; METASTASIS; MICRORNA; PHOSPHATASES; REGRESSION analysis; EPITHELIAL-mesenchymal transition; CELL proliferation; PHOSPHOPROTEINS; CELL lines
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2023, Vol 2023, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2023/5177471