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- Title
Developmental arrest of scNT-derived fetuses by disruption of the developing endometrial gland as a result of impaired trophoblast migration and invasiveness.
- Authors
Kim, Jae-Hwan; Park, Jong-Yi; Park, Mi-Rung; Hwang, Kyu-Chan; Park, Keun-Kyu; Park, Chankyu; Cho, Seong-Keun; Lee, Hwi-Cheul; Song, Hyuk; Park, Soo-Bong; Kim, Teoan; Kim, Jin-Hoi
- Abstract
Somatic cell nuclear transfer (scNT)-derived pig placenta tissues of gestational day 30 displayed avascularization and hypovascularization. Most of the cytotrophoblast-like cells of the developing scNT-derived placenta villi were improperly localized or exhibited impaired migration to their targeting loci. Id-2, Met, MMP-9, and MCM-7 were barely detectable in the cytotrophoblast cells of the scNT-derived placenta villi. Active MMP-2 and MMP-9 expression was significantly down-regulated in the scNT-embryo transferred recipient uteri. scNT clones exhibited a hypermethylated pattern within the pig MMP-9 promoter region and the significance of GC box in the regulation of MMP-9 promoter activity. Marked apoptosis was observed in the developing endometrial gland of scNT-embryo transferred recipient uteri. Collectively, our data strongly indicated that early gestational death of scNT clones is caused, at least in part, by disruption of the developing endometrial gland as a result of impaired trophoblast migration and invasiveness due to the down-regulation of active MMP-9 expression. Developmental Dynamics 240:627-639, 2011. © 2011 Wiley-Liss, Inc.
- Publication
Developmental Dynamics, 2011, Vol 240, Issue 3, p627
- ISSN
1058-8388
- Publication type
Article
- DOI
10.1002/dvdy.22568