We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Label-Free Spectral Imaging Unveils Biochemical Mechanisms of Low-Level Laser Therapy on Spinal Cord Injury.
- Authors
Yuze Gong; Shuang Wang; Zhuowen Liang; Zhe Wang; Xu Zhang; Jie Li; Jiwei Song; Xueyu Hu; Kaige Wang; Qingli He; Jintao Bai
- Abstract
Background/Aims: Low-level laser therapy (LLLT) leads to complex photochemical responses during the healing process of spinal cord injury (SCI). Confocal Raman Microspectral Imaging (in combination with multivariate analysis) was adopted to illustrate the underlying biochemical mechanisms of LLLT treatment on a SCI rat model. Methods: Using transversal tissue sections, the Raman spectra can identify areas neighboring the injury site, glial scar, cavity, and unharmed white matter, as well as their correlated cellular alterations, such as demyelination and up-regulation of chondroitin sulfate proteoglycans (CSPGs). Multivariate data analysis methods are used to depict the underlying therapeutic effects by highlighting the detailed content and distribution variations of the biochemical constituents. Results: It is confirmed that photon-tissue interactions might lead to a decay of the inhibitory response to remyelination by suppressing CSPG expression, as also morphologically demonstrated by reduced glial scar and cavity areas. An inter-group comparison semi-quantitatively confirms changes in lipids, phosphatidic acid, CSPGs, and cholesterol during SCI and its LLLT treatment, paving the way for in vitro and in vivo understanding of the biochemical changes accompanying pathobiological SCI events. Conclusion: The achieved results in this work not only have once again proved the well-known cellular mechanisms of SCI, but further illustrate the underlying biochemical variability during LLLT treatment, which provide a sound basis for developing real-time Raman methodologies to monitor the efficacy of the SCI LLLT treatment.
- Subjects
SPECTRAL imaging; SPINAL cord injuries; MYELINATION; MEDICAL lasers; RAMAN spectra; LABORATORY rats; THERAPEUTICS
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2018, Vol 49, Issue 3, p1168
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000493295