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- Title
Differential Effects of 20 Non–Dioxin-Like PCBs on Basal and Depolarization-Evoked Intracellular Calcium Levels in PC12 Cells.
- Authors
Langeveld, Wendy T.; Meijer, Marieke; Westerink, Remco H.S.
- Abstract
Non–dioxin-like polychlorinated biphenyls (NDL-PCBs) are environmental pollutants that are well known for their neurotoxic effects. Numerous in vitro studies reported PCB-induced increases in the basal intracellular calcium concentration ([Ca2+]i), and in vivo NDL-PCB neurotoxicity appears at least partly mediated by these disturbances. However, effects of NDL-PCBs on depolarization-evoked calcium influx are poorly investigated, and effects of several congeners, including PCB53, on calcium homeostasis are still unknown. We therefore studied the effects of 20 selected NDL-PCBs on basal and depolarization-evoked [Ca2+]i in fura-2–loaded PC12 cells using single-cell fluorescence microscopy. The results demonstrate that hexa- and heptachlorobiphenyls (with the exception of PCB136) were unable to affect basal and depolarization-evoked [Ca2+]i. However, most tri- and tetrachlorinated as well as some pentachlorinated NDL-PCBs (at 1 and 10μM) increased basal [Ca2+]i during a 15-min exposure. The increase in basal [Ca2+]i, which differed in kinetics for the different congeners, depended partly on influx of extracellular calcium and calcium release from the endoplasmic reticulum. Importantly, all tested tri- and tetrachlorinated biphenyls and some pentachlorinated NDL-PCBs (PCB95, PCB100, and PCB104) reduced depolarization-evoked [Ca2+]i, with PCB51 and PCB53 being most potent (near complete inhibition at 1μM). The reduction in depolarization-evoked calcium influx depended on the exposure duration but not on the foregoing PCB-induced increase in basal [Ca2+]i. The inhibition of voltage-gated calcium channels is a novel and sensitive mode of action for NDL-PCBs that contributes to the disturbances in calcium homeostasis and likely is related to NDL-PCB–induced (developmental) neurotoxicity.
- Subjects
NEUROTOXICOLOGY; POLYCHLORINATED biphenyls; DIOXINS; INTRACELLULAR calcium; POLLUTANTS; CALCIUM channels; HOMEOSTASIS; ENDOPLASMIC reticulum
- Publication
Toxicological Sciences, 2012, Vol 126, Issue 2, p487
- ISSN
1096-6080
- Publication type
Article
- DOI
10.1093/toxsci/kfr346