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- Title
Expression of Tyrosine Hydroxylase Increases the Resistance of Human Neuroblastoma Cells to Oxidative Insults.
- Authors
Franco, Jeferson L.; Posser, Thaís; Gordon, Sarah L.; Bobrovskaya, Larisa; Schneider, Jennifer J.; Farina, Marcelo; Dafre, Alcir L.; Dickson, Phillip W.; Dunkley, Peter R.
- Abstract
In this study, we demonstrate that human neuroblastoma SH-SY5Y cells transfected with human tyrosine hydroxylase isoform 1 (SH + TH cells) were substantially more resistant to cell death induced by hydrogen peroxide and 6-hydroxydopamine when compared to wild-type SH-SY5Y cells (SH cells). SH + TH cells exhibit increased levels of dopamine (DA) compared to SH cells. Incubation with hydrogen peroxide or 6-hydroxydopamine (10–100μM) for 24 h caused a significant reduction in cell viability and increased apoptosis in both cell types. However, these effects were significantly reduced in the SH + TH cells when compared to the SH cells. The SH + TH cells showed an improved ability to detoxify peroxide, which correlated with an increase in glutathione peroxidase and glutathione reductase activities, while catalase activity was unchanged. Our data suggest that a preconditioning-like mechanism linked to higher DA levels increased the resistance of SH + TH cells against oxidative insults, which is at least in part related to an augmentation in the activity of glutathione-related antioxidant enzymes.
- Subjects
TYROSINE; NEUROBLASTOMA; DOPAMINE; HYDROGEN peroxide; DETOXIFICATION (Alternative medicine); ANTIOXIDANTS
- Publication
Toxicological Sciences, 2010, Vol 113, Issue 1, p150
- ISSN
1096-6080
- Publication type
Article
- DOI
10.1093/toxsci/kfp245