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- Title
Changes of Serum Inflammatory Markers after Gamma-Secretase Inhibitor Administration Mice.
- Authors
Khazaei, Majid; Saeidi, Hajar; Shabanikia, Niloofar; Kalantari, Elmira; Tahergorabi, Zoya
- Abstract
Background: Notch signaling is involved in physiological and pathological processes and gammasecretase inhibitors can suppress it. The aim of this study was to evaluate the effect of DAPT, a gamma secretase inhibitor, on serum inflammatory markers in mice. Methods: 24 C57BL6 mice (age: 5 weeks old) were randomly divided into control and obese groups. Obese group received high-fat diet (60% fat, 20% protein, and 20% carbohydrate) for 20 weeks. Control group received standard diet during this time. Then, each group was divided into receiving and non-receiving DAPT (50mg/kg; Subcutaneous). After 14 days, blood samples were taken for measurements of serum high-sensitive C-reactive protein (hs-CRP) and interlukin-6 (IL-6). Findings: There was no significant difference in serum hs-CRP concentration between control and obese groups (P > 0.05). Administration of DAPT did not alter serum hs-CRP level in both groups (P > 0.05). Serum Il-6 was significantly higher in obese animals compare to controls (P < 0.05) and administration of DAPT reduced serum IL-6 in control and obese animals (P < 0.05). Conclusion: DAPT, as a gamma secretase inhibitor, could affect several physiological and pathological vascular changes by alteration of serum inflammatory markers.
- Subjects
BIOMARKERS; DRUG administration; SECRETASE inhibitors; CONCENTRATION functions; HIGH-fat diet; C-reactive protein
- Publication
Journal of Isfahan Medical School, 2013, Vol 31, Issue 233, p486
- ISSN
1027-7595
- Publication type
Article