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- Title
Upconversion optogenetic micro-nanosystem optically controls the secretion of light-responsive bacteria for systemic immunity regulation.
- Authors
Yang, Chun; Cui, Meihui; Zhang, Yingying; Pan, Huizhuo; Liu, Jing; Wang, Shixing; Ma, Ning; Chang, Jin; Sun, Tao; Wang, Hanjie
- Abstract
Chemical molecules specifically secreted into the blood and targeted tissues by intestinal microbiota can effectively affect the associated functions of the intestine especially immunity, representing a new strategy for immune-related diseases. However, proper ways of regulating the secretion metabolism of specific strains still remain to be established. In this article, an upconversion optogenetic micro-nanosystem was constructed to effectively regulate the specific secretion of engineered bacteria. The system included two major modules: (i) Modification of secretory light-responsive engineered bacteria. (ii) Optical sensing mediated by upconversion optogenetic micro-nanosystem. This system could regulate the efficient secretion of immune factors by engineered bacteria through optical manipulation. Inflammatory bowel disease and subcutaneously transplanted tumors were selected to verify the effectiveness of the system. Our results showed that the endogenous factor TGF-β1 could be controllably secreted to suppress the intestinal inflammatory response. Additionally, regulatory secretion of IFN-γ was promoted to slow the progression of B16F10 tumor. Yang, Cui, Zhang et al. report an optogenetic nanosystem which controls the secretion of immune factors by engineered light-responsive bacteria for systemic immunity regulation. On shining NIR light on mice's stomach, blue light emission from upconversion rods induce excretion of TGF-β1 and IFN-γ from the bacteria, thereby suppressing intestinal inflammatory response or slowing down progress of subcutaneously transplanted tumour, respectively.
- Subjects
EFFECT of light on bacteria; OPTOGENETICS; INFLAMMATORY bowel diseases; IMMUNE response; TRANSFORMING growth factors-beta
- Publication
Communications Biology, 2020, Vol 3, Issue 1, pN.PAG
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-020-01287-4