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- Title
基于网络药理学研究汉黄芩素治疗糖尿病 视网膜病变的机制.
- Authors
侯亚莉; 梅 稳; 郭菲菲; 贺明娟; 吴国琼; 夏 婷; 林 梅
- Abstract
Objective To predict the targets and signaling pathways of wogonin in the treatment of diabetic retinopathy (DR), to preliminarily verify the related targets through cell experiments, and to elucidate the mechanism of wogonin on DR. Methods The targets of wogonin were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) database, and the targets related to DR were screened by Comparative Toxicogenomics Database (CTD), Human Gene Function And Network Analysis Database (GenCLiP), DisGeNET and GeneCards databases. The protein interaction network (Protein-Protein-Interaction-Network, PPI) was analyzed by STRING database and Cytoscape 3.6.1 software. The Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signal pathway enrichment analysis were carried out by DAVID. Finally, the core target and pathway of wogonin were preliminarily verified by the experimental model of ARPE-19 cytopathy induced by high concentration of D-glucose intervened by wogonin. The cell morphology was observed under light microscope, the cell viability was detected by Cell Counting Kit-8 (CCK8) assay, and the expression of target genes was analyzed by Real Time Quantitative Polymerase Chain Reaction (RT-qPCR). Results A total of 204 possible potential targets of wogonin, 313 targets related to DR and 30 core targets after the interaction between wogonin targets and DR targets were identified. GO enrichment analysis showed that wogonin was mainly involved in response to osmotic stress, membrane raft, insulin receptor substrate binding and other biological processes. KEGG enrichment analysis showed that Advanced Glycation End-Advanced Glycation End Receptor (AGE-RAGE) in diabetic complications, Endocrine resistance, Epidermal Growth Factor Receptor (EGFR) tyrosine kinase inhibitor resistance, Interleukin-17 (IL-17) and other signaling pathways were more enriched. Finally, Protein kinase B (AKT1), B-cell lymphoma 2 (BCL2) and Caspase-3 (CASP3) in AGE-RAGE signaling pathway were successfully verified as important targets by ARPE-19 cell in vitro, which suggested that wogonin had a significant improvement effect on DR. Conclusion Wogonin exerts the therapeutic effect on DR through multi-targets, multi-pathways by network pharmacology, and more the core targets in AGE-RAGE signaling pathway are successfully verified by cells, providing the scientific basis for further elucidation of the mechanism of wogonin in the treatment of DR.
- Publication
Journal of Shenyang Pharmaceutical University, 2023, Vol 40, Issue 10, p1456
- ISSN
1006-2858
- Publication type
Article
- DOI
10.14066/j.cnki.cn21-1349/r.2021.0494