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- Title
Güney Marmara Bölgesi kabakgil üretim alanlarında cucumber mosaic virus enfeksiyonunun tespiti ve kılıf protein gen diziliminin filogenetik analizi.
- Authors
KARANFİL, Ali; KORKMAZ, Savaş
- Abstract
Objective: This study was carried out for the detection and molecular characterization of cucumber mosaic virus (CMV) infecting cucurbit plants in the Southern Marmara Region (SMR) of Turkey. Material and Methods: Surveys were performed in the SMR (Çanakkale, Balıkesir and Bursa provinces) and, samples showing virus and virus-like symptoms were collected. The samples were tested by DAS-ELISA to detect CMV infection. For further analysis, three isolates were selected according to the geographic origin which they were obtained from. Their complete coat protein genes were amplified by RT-PCR, cloned and sequenced. Results: While seventy-two samples were collected, CMV infection was found in 10 plants. As a result of sequence analysis, SMR CMV isolates were 93.15-99.70% and 97.71-100% similar to each other at nucleotide (nt) and amino acid (aa) levels, respectively. And, it was determined that 76-100% similarities with world isolates at nt and aa levels. Moreover, based on the phylogenetic analysis, it was determined that two of the GMB CMV isolates were in subgroup IA and remaining was in subgroup IB. Conclusion: CMV infections were detected in cucurbit plant production areas of SMR. And, for the first time, complete coat protein gene sequences of Turkish cucurbit CMV isolates were deposited in Genbank.
- Subjects
CANAKKALE (Turkey); AMINO acid sequence; SYMPTOMS; SEQUENCE analysis; AMINO acids; COAT proteins (Viruses); VIRUSES; CYTOMEGALOVIRUS diseases
- Publication
Ege Üniversitesi Ziraat Fakültesi Dergisi, 2021, Vol 58, Issue 2, p239
- ISSN
1018-8851
- Publication type
Article
- DOI
10.20289/zfdergi.682293