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- Title
Effect of Trehalose on Cryopreservation of Hariana Bull Spermatozoa.
- Authors
Kumar, Gyanesh; Yadav, Dileep Kumar; Saxena, Atul; Sachan, Vikas; Kumar, Anuj; Swain, Dilip Kumar; Yadav, Brijesh
- Abstract
The present experiment was designed to study the effect of supplementation of Trehalose on freezability and antioxidant activity of Hariana bull semen. Semen samples were diluted in TFYG extender containing different concentrations of Trehalose (10 mM, 30 mM & 50 mM). The control samples were extended with TFYG alone. Results clearly indicated that, 30 mM Trehalose group had significantly (P<0.05) higher percentage of individual sperm motility, sperm viability, and intact acrosome in comparison to the control and other Trehalose supplemented groups. In biochemical assays, no clear-cut demarcation for antioxidant enzyme was found for Trehalose supplementation. However, Trehalose supplementation has showed increase activity for Glutathione reductase (GR). The results obtained clearly indicated that supplementation of Trehalose (30 mM) to extender prior to cryopreservation improves Hariana bull sperm quality.
- Subjects
HARYANA (India); TREHALOSE; SPERMATOZOA; GLUTATHIONE reductase; BULLS; SPERM motility
- Publication
Indian Journal of Veterinary Sciences & Biotechnology, 2023, Vol 19, Issue 6, p8
- ISSN
2394-0247
- Publication type
Article
- DOI
10.48165/ijvsbt.19.6.02