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- Title
Insidious transmission of Mycobacterium tuberculosis in Ordos, China: a molecular epidemiology study.
- Authors
Sun, Hailin; Ma, Zichun; Ai, Fuli; Han, Bing; Li, Peng; Liu, Juan; Wu, Yiheng; Wang, Yufeng; Li, Bing; Qi, Dan; Pang, Yu
- Abstract
Background: In this study, we conducted this population-based study to evaluate the genetic diversity and clustering rate of Mycobacterium tuberculosis (MTB) strains using the whole-genome sequencing (WGS), to better understand its transmission in Ordos. Methods: All patients with culture-positive TB notified in Ordos from January 2021 to December 2022 were recruited. WGS was performed to analyze single-nucleotide polymorphism (SNP) and to identify genotypic drug susceptibilities of MTB isolates. Results: Overall, a total of 186 patients were included in the present study, of whom 35 (18.8%) had no symptoms suggestive of active TB. Lineage 2 was the predominant MTB sublineage, accounting for 186 of isolates tested. When the pairwise SNP difference ≤ 12 was used as the cutoff for WGS-based clusters, we identified 17 genotypic clusters, and 38 isolates belonged to these 17 clusters, resulting in a clustering rate of 20.4%. The Beijing genotype was an independent factor associating with genomic-clustering (adjusted OR 4.219, 95% CI 0.962–18.502). The overall sensitivity on WGS-based resistance prediction was 85.7% for rifampicin, 73.1% for isoniazid, 60.0% for Ethambutol, 72.7% for streptomycin, and 72.7% for fluoroquinolones. Conclusion: To conclude, the present study demonstrates the extensive recent transmission of Beijing genotype strains in the community of Ordos. The failure to provide a comprehensive pattern of transmission indicated the missed diagnosis of active TB within the community. A substantial proportion of subclinical TB cases are recognized in the bacteria-positive cases, emphasizing that we must interrupt transmission by finding people with active TB before they infect others.
- Subjects
ORDOS Desert (China); BEIJING (China); MYCOBACTERIUM tuberculosis; MOLECULAR epidemiology; WHOLE genome sequencing; TUBERCULOSIS; SINGLE nucleotide polymorphisms; GENETIC variation
- Publication
European Journal of Clinical Microbiology & Infectious Diseases, 2024, Vol 43, Issue 2, p305
- ISSN
0934-9723
- Publication type
Article
- DOI
10.1007/s10096-023-04730-6