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- Title
Protective eff ects of zinc chloride on azathioprineinduced toxicity on germinal epithelium of albino rats: A morphometric study.
- Authors
Shaikh, Shahid Pervez; Mohiuddin, Maria; Ali, Amjad; Qamar, Zeeshan; Inayatullah; Kumar, Hemant
- Abstract
Objective: To determine the eff ects of azathioprine on germinal epithelium and evaluate the possible protection provided by co-administration of zinc chloride. Design: A prospective experimental study. Place of study: Anatomy Department, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi from January 2016 till January 2017. Material and Methods: For this study 60-male adult albino rats of 90-120 days of age were selected and distributed into three equal groups A, B and C. Th ey were further divided into four sub-groups for 1st, 3rd, 6th and 8th weeks of the treatment. Group-A served as control, received only injection 0.9 % normal saline intraperitoneally daily, group-B received Inj. azathioprine 15mg/kg body weight intra-peritoneally daily and group-C received Inj. azathioprine 15mg/ kg body weight plus Inj. zinc chloride 1mg/kg body weight intra-peritoneally daily. At the end of respective period of treatment animals were sacrifi ced. Both testes were excised, fi xed in Bouin's solution for 24 hours. Aft er processing in ascending grades of alcohol, cleared in xylene and fi xed in paraffi n, 5 μ thick sections were cut and stained with PAS-Iron Haematoxylin for study of seminiferous tubules morphology and micrometry. Results: Th e tubules were compact, regularly arranged with intact basement membrane. Th e lumens of germinal epithelium contain mature spermatozoa in control group-A. Th e spaces between seminiferous tubules were increased with area of necrosis, the basement membrane of most of tubules were distorted and ruptured, the vacuoles between the germinal epithelium were appeared and the lumen contain slough without spermatozoa in group-B. Th ere was widening of intertubular spaces, the tubules were wavy but basement membrane intact. Lumens were narrow and contained spermatozoa without slough in group-C, otherwise features similar to control. Conclusion: Azathioprine produced germinal epithelium toxicity which is more pronounced with time period and provided protection by zinc chloride.
- Subjects
KARACHI (Pakistan); ZINC chloride; MEDICAL sciences; EPITHELIUM; BASAL lamina; SEMINIFEROUS tubules
- Publication
Pakistan Journal of Surgery, 2020, Vol 36, Issue 1, p83
- ISSN
0258-8552
- Publication type
Article