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- Title
Physiological and morphological evidence of brassinosteroid-biosynthesis inhibition by the fungicide imazalil.
- Authors
Werbrouck, Stefaan P. O.; Saibo, Nelson J. M.; Dhuyvetter, Hilde; De Schepper, Sandra; Van Der Straeten, Dominique; Debergh, Pierre C.
- Abstract
Dark germinated Arabidopsis thaliana Ler seedlings grown on medium with the commonly used imidazole-type fungicide imazalil (IMA) resemble de-etiolated (det ) brassinosteroid-deficient mutants. IMA hampers cell elongation in the hypocotyl, but stimulates radial expansion during dark growth. This phenotype could partially be restored by simultaneous addition of 24-epibrassinolide (EBR). A complete restoration of the hypocotyl length could only be achieved by combining EBR and gibberellic acid. As Arabidopsis thaliana etr1-3 de-etiolates on IMA containing medium in the dark, in the same dose-dependent manner as the wild type, its effects cannot be attributed to the induction of extra ethylene production. Studies with A. thaliana seedlings expressing CPD::GUS show that IMA up-regulates the expression of CPD, which encodes a key cytochrome P450 enzyme in the brassinosteroid (BR)-biosynthesis pathway. This effect is reverted by EBR, indicating that the up-regulation of CPD by IMA might result from the lack of end product brassinolide. Together these data suggest that, in Arabidopsis , one of the effects of IMA is an inhibition of BR-biosynthesis. IMA is an available and cheap agrochemical that might be a valuable tool for future brassinosteroid research.
- Subjects
SEEDLINGS; FUNGICIDES; PLANT physiology
- Publication
Physiologia Plantarum, 2003, Vol 119, Issue 1, p69
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1034/j.1399-3054.2003.00155.x