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- Title
The clinical significance of antibody to vascular endothelial cells after renal transplantation.
- Authors
Nakagawa, Yuki; Saito, Kazuhide; Morioka, Testuo; Tomita, Yoshihiko; Takahashi, Kota; Oite, Takashi
- Abstract
Abstract: Vascular endothelial cells (ECs) are considered to be a primary target for injury in allograft rejection. However, the relationship between serum antibody activity to ECs and rejection episodes has not been examined extensively in renal transplantation. Twenty-two renal transplant recipients were included in this study. Serum antibody activity to vascular endothelial cells (AECA) was measured using a cellular enzyme-linked immunosorbent assay (ELISA) in which human umbilical vein endothelial cells (HUVEC) and human glomerular endothelial cells (HGEC) were preincubated with TNF-α used as target cells. Serum samples were obtained just before transplantation and once a week during the immediate 1–3-month post-transplantation period. There was a significant correlation between the presence of AECA against HGEC and rejection episodes (P < 0.05). Patients with multi-episodes of rejection showed significantly higher frequencies of AECA than patients with mono-episodic rejection (P < 0.0005). It should be noted that patients suffering from multi-episodes of rejection revealed higher AECA titres before transplantation. These findings imply that the HGEC–ELISA could be used as a prospective, informative test to identify patients with a higher risk of acute rejection in renal transplantation.
- Subjects
VASCULAR endothelium; KIDNEY transplantation; ENDOTHELIUM; IMMUNOGLOBULINS
- Publication
Clinical Transplantation, 2002, Vol 16, p51
- ISSN
0902-0063
- Publication type
Article
- DOI
10.1034/j.1399-0012.16.s8.10.x