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- Title
Transposon Display identifies individual transposable elements in high copy number lines.
- Authors
Va, Dirk; de, n; Broeck, n; Maes, Tamara; Sauer, Matt; Zethof, Jan; D, Peter; Keukeleire, e; D’hauw, Mariëlla; Va, Marc; Montagu, n; Gerats, Tom
- Abstract
Summary: The dTph1 transposable element family of Petunia hybrida line W138 consists of between 100 and 200 members. A strategy that allows simultaneous detection of individual elements is described. Sequences flanking dTph1 elements are amplified by means of a ligation-mediated PCR. The resulting fragments are locus-specific and can be analysed by polyacrylamide gel electrophoresis. One of the applications of Transposon Display is the isolation of dTph1-tagged genes. Fragments that co-segregate with a mutant phenotype can be extracted from the gel and reamplified, providing access to tagged genes, as demonstrated in a reconstruction experiment. Data on the molecular identification of a phenotypic mutant, isolated in a random tagging experiment is also presented. Upon sequencing, the obtained candidate fragment was found to be identical to part of the previously identified Fbp1 gene.
- Subjects
CHROMOSOMAL translocation; TRANSPOSONS
- Publication
Plant Journal, 1998, Vol 13, Issue 1
- ISSN
0960-7412
- Publication type
Article