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- Title
Expression of MHC class I receptors confers functional intraclonal heterogeneity to a reactive expansion of γδ T cells.
- Authors
Lafarge, Xavier; Pitard, Vincent; Ravet, Sophie; Roumanes, David; Halary, Franck; Dromer, Claire; Vivier, Eric; Paul, Pascale; Moreau, Jean-François; Déchanet-Merville, Julie
- Abstract
NK cell receptors for MHC class I molecules (MHC-NKR) can be expressed by T cell subsets. The restricted repertoire and phenotypic characteristics of MHC-NKR T cells indicate that expression of MHC-NKR is acquired upon antigenic challenge and might promote expansion of T cells. Previous studies performed on in vitro generated αβ T cell clones concluded that MHC-NKR expression was not a clonal attribute. Here, we examined a massive monoclonal expansion of a non-leukemic γδ T cell population found in the peripheral blood of a lung-transplanted patient who suffered from a cytomegalovirus infection. Despite their monoclonality, these T cells displayed a heterogeneous and stable in vivo Ig- and lectin-like MHC-NKR phenotype. Twenty percent of the cells displayed a CD94NKG2A phenotype, and 10% were labeled with an anti-CD158b1/b2/j monoclonal antibody. A CD158b/j γδ T cell clone derived in vitro from patient's peripheral blood lymphocytes was shown to express the activating form CD158j (KIR2DS2), which once cross-linked stimulated the clone cytolytic function and costimulated the TCR-induced production of cytokines, independently of the killer-activating receptor-associated protein (KARAP). In conclusion, heterogeneity of MHC-NKR expression confers a functional intraclonal diversity that may participate to induction of specific γδ T cell effector functions or proliferation upon pathogen challenge.
- Publication
European Journal of Immunology, 2005, Vol 35, Issue 6, p1896
- ISSN
0014-2980
- Publication type
Article
- DOI
10.1002/eji.200425837