We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Improvement of Sleeping Beauty Transposon System Enabling Efficient and Stable Protein Production.
- Authors
Lee, Yun Haeng; Park, Ji Yun; Song, Eun Seon; Lee, Haneur; Kuk, Myeong Uk; Joo, Junghyun; Roh, Hyungmin; Park, Joon Tae
- Abstract
The most essential goal in the field of biopharmaceuticals is to develop cell lines with higher protein yields. To this goal, the Sleeping Beauty (SB) transposon-based expression system has been developed as a powerful tool for increasing protein productivity. However, SB transposon system has fallen short of expectation in terms of the efficiency and stability of protein production, limiting its applicability to large-scale production of recombinant proteins. Here, we propose a novel strategy to increase the efficiency and stability of protein production, through modification of the traditional SB transposon vector. Adding a pair of inverted terminal repeats (ITRs) next to existing ITRs (i.e., double-ITRs) significantly increased the efficiency of transgene integration, resulting in high-yield and sustained protein production. Furthermore, double-ITRs responded more favorably to DNA methylation inhibitors in terms of protein yield, implying that using double-ITRs with DNA methylation inhibitors may be effective in increasing protein productivity. Taken together, our study introduces a new vector platform that is applicable to high-yield and sustained protein production, and will open new avenues in the field of biopharmaceuticals.
- Subjects
TRANSPOSONS; RECOMBINANT proteins; PROTEIN stability; DNA methylation; PROTEINS
- Publication
Biotechnology & Bioprocess Engineering, 2022, Vol 27, Issue 3, p353
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-021-0231-x