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- Title
An in vitro Study for the Synergistic Cytotoxic Actions of Biological Therapy and Anticancer Chemotherapy.
- Authors
Obayes, Sarah K.; Obied, Hamid Naji; Al-Saadi, Mohammed A. K.
- Abstract
Objective: This study aimed to investigate the increase of the cytotoxic action of a minimal dose of anticancer chemotherapy (temozolomide) in combination with the biological therapy (interferon-alpha-2a). Seven in vitro experiments were used in this study. The cytotoxicity of temozolomide, interferon-alpha-2a and the combination of them was measured on melanoma B16 cell line for 24 and 48 hours incubation periods. The cells of these lines were seeded in 96-well plate. Temozolomide was used in a six serial dilutions (6.25, 12.5, 25, 50, 100, 200) μg/ml. Interferon-alpha-2a was also used in a six serial dilutions (37500, 75000, 125000, 250000, 500000, 1000000) IU/ml. The MTT(3-(4,5-Dimethylthiazole-2-yl)-2,5-diphenyl- 2H-tetrazolium bromide) assay was used to determine the number of viable cells and the intensity of color was measured by ELISA reader. The combination of temozolomide and interferon-alpha-2a at 48 hours incubation period, there was a significant decrease (P≤ 0.05) in the viability percent of melanoma B16 cells for all concentrations of the combination therapy as compared to the control group.
- Subjects
BIOTHERAPY; CANCER chemotherapy; IN vitro studies; TEMOZOLOMIDE; DACARBAZINE; CELL lines
- Publication
Indian Journal of Forensic Medicine & Toxicology, 2019, Vol 13, Issue 3, p237
- ISSN
0973-9122
- Publication type
Article
- DOI
10.5958/0973-9130.2019.00202.0