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- Title
Microbiota of MR1 deficient mice confer resistance against Clostridium difficile infection.
- Authors
Smith, Ashley D.; Foss, Elissa D.; Zhang, Irma; Hastie, Jessica L.; Giordano, Nicole P.; Gasparyan, Lusine; VinhNguyen, Lam Phuc; Schubert, Alyxandria M.; Prasad, Deepika; McMichael, Hannah L.; Sun, Jinchun; Beger, Richard D.; Simonyan, Vahan; Cowley, Siobhán C.; JrCarlson, Paul E.
- Abstract
Clostridium difficile (Cd) infection (CDI) typically occurs after antibiotic usage perturbs the gut microbiota. Mucosa-associated invariant T cells (MAIT) are found in the gut and their development is dependent on Major histocompatibility complex-related protein 1 (MR1) and the host microbiome. Here we were interested in determining whether the absence of MR1 impacts resistance to CDI. To this end, wild-type (WT) and MR1-/- mice were treated with antibiotics and then infected with Cd spores. Surprisingly, MR1-/- mice exhibited resistance to Cd colonization. 16S rRNA gene sequencing of feces revealed inherent differences in microbial composition. This colonization resistance was transferred from MR1-/- to WT mice via fecal microbiota transplantation, suggesting that MR1-dependent factors influence the microbiota, leading to CDI susceptibility.
- Subjects
CLOSTRIDIOIDES difficile; FECAL microbiota transplantation; MICE; BACTERIAL spores; GUT microbiome
- Publication
PLoS ONE, 2019, Vol 14, Issue 9, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0223025