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- Title
<em>In situ</em> expression and soluble form of P-selectin in human glomerulonephritis.
- Authors
Segawa, Chikako; Wada, Takashi; Takaeda, Masayoshi; Furuichi, Kengo; Matsuda, Ichirou; Hisaida, Yukimasa; Ohta, Satoshi; Takasawa, Kazuya; Takeda, Shin-ichi; Kobayashi, Ken-ichi; Yokoyama, Hitoshi
- Abstract
To clarify the early involvement of cellular adhesion molecules in human glomerulonephritis. we investigated P-selectin and high endothelial venules' (HE'Vs) marker MECA-79 expression in kidney specimens by immunohistochemical and<em> in situ</em> hybridization analyses, arid measured serum and urinary soluble P-selectin levels by enzyme-linked immunosorbent assay. In normal controls, P-selectin and MECA-79 expression were negative in glomeruli (N = 4). and serum soluble P-selectin levels were 114.3 ± 36.8 ng/ml (mean ± SEM, (<em>N</em> = 12). Soluble P-selectin was not detectable in urincof all cases. In proliferative glomerutonephritis involving rapidly progressive glomerulonephrtis (<em>N</em> = 6) IgA nephropathy (<em>N</em> = 26) lupus nephritic (<em>N</em> = 7) and acute glomerulonephritis (<em>N</em> =2) both glomerular and interstitial P-sellectin expression were up-regulated Glomerular P-selectin expression correlated positively with local cellular. accumulation endocapillary, proliferation and CD41b (platelet) staining interstitial P-selectin expression showed a positive correlation with the grade of local cellular infiltrates P-seletin mRNA signals detected by <em>in situ</em> hybridization were only observed on capillary or venous endothelium in the interstitium but not in glomeruli. In addition, MECA-79 was expressedon the plump endothelial cells at the cortico-medullary junction (outer medulla) Serum soluble P-selectin levels were significantly higher in patients with proliferative gIomerulonephritis especially in glomerular and interstitial P-selectin positive staining, and correlated with glomerular endocapillary proliferation. These observations suggested that P-selectin was associated with both glomerular and interstitial leukonte accumulation in human glomerulonephritis. and niight he expressed by two distinct mechanisms that are the activated platelets in glomeruli and the <em>de novo</em> :expression in the interstitial lesions that correlatedwith MECA-79 expression as HEVs like vessels, and serum soluble P-sclcctin may he a useful marker for predicting <em>in situ</em> P-selectin expression associated with glomerular endocapillary proliferation in nephritis.
- Subjects
GLOMERULONEPHRITIS; CUTANEOUS tuberculosis; EPITHELIUM; LEUCOCYTES; PATIENTS; IMMUNE complex diseases
- Publication
Kidney International, 1997, Vol 52, Issue 4, p1054
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1997.428