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- Title
Peritoneal dialysis solutions reverse the hemodynamic effects of nitric oxide synthesis inhibitors.
- Authors
White, Randall; Barefield, Darren; Ram, Sunanda; Work, Jack
- Abstract
Nitric oxide (NO) synthesis is inhibited by a variety of L-arginine an-dogs including NG-nitro-L-arginine methyl ester (L-NAME) and NG-NG-dimethylarginine (ADMA). ADMA is present in elevated concentrations in renal failure and potentially could alter microcirculatory hemodynamics during peritoneal dialysis (PD). This investigation utilized the techniques of intravital microscopy to quantitate the mesenteric arteriolar hemodynamic effects of PD solutions during NO synthesis inhibition. L-NAME (100 μM) produced maximum arteriolar vasoconstriction to 74% of baseline diameter (19.9 ± 2.2 vs. 26.9 ± 1.4 μm, P < 0.001, N = 10) and ADMA (100 μ) to 68% (20.5 ± 2.5 vs. 30.1 = 6). L-NAME decreased red blood cell velocity to 44% of baseline velocity (3.8 ± 0.8 vs. 8.5 ± 1.1 mm/second, P < 0.001) and ADMA to 52% (5.1 ± 1.1 vs. 9.8 ± 0.9 mm/second, P < 0.01, N = 6). Despite NO synthesis inhibition, standard PD solutions reversed these hemodynamic effects with both 1.5% and 4.25% Dianeal (Baxter) rapidly reversing the vasoconstriction and restoring blood flow back to baseline values. When Dianeal and L-NAME were simultaneously superfused, no L-NAME induced vasoconstriction occurred and Dianeal maintained vasodilatory properties despite L-NAME (P < 0.01, N = 5). This investigation reaffirms that basal levels of NO are important in maintaining normal hemodynamics in the mesenteric microcirculation. Reversal of the L-NAME induced arteriolar hemodynamic effects by Dianeal suggests that the endogenous NO synthesis inhibitor ADMA has no significant effects in the regulation of the mesenteric microvascular arteriolar hemodynamics during PD. Since these PD solutions remain vasoactive despite NO synthesis inhibition, this suggests that these PD solutions possess vasoactive properties primarily through a NO independent mechanism.
- Subjects
PERITONEAL dialysis; HEMODIALYSIS; DIALYSIS (Chemistry); THERAPEUTICS; NITRIC oxide; NITROGEN compounds; KIDNEY diseases
- Publication
Kidney International, 1995, Vol 48, Issue 6, p1986
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1995.501