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- Title
Isolation by monoclonal antibody of intercalated cells of rabbit kidney.
- Authors
Koseki, Chizuko; Yamaguchi, Yasunori; Furusawa, Mitsuru; Endou, Hitoshi
- Abstract
We produced a monoclonal antibody, γG6, that reacts only with one cell type in the connecting (CNT) and collecting tubules (CT) of rabbit kidney. The γG6 antibody-reactive cells .revealed carbonic anhydrase activity, showing one of the characteristics of intercalated (IC) cells. Using immunoelectron microscopy, we demonstrated that IC cells in comical CT consist of the γG6 antibody-reactive and non-reactive cells, whereas all IC cells in medullary CT were reactive with the γG6 antibody. We used a cell sorter to enrich this cell type from the isolated kidney cell suspension. When we measured hormone-sensitive adenylate cyclase (ACase) activities of the sorted cells, the presence of parathyroid hormone (PTH) and isoproterenol (ISO) almost doubled ACase activities when compared with the basal values: however, no additive effect of PTH and ISO was observed. They showed no calcitonin-sensitive ACase and negligible arginine vasopressin (AVP)-sensitive ACase. We suggest that the IC cells recognized by the γG 6 monoclonal antibody possess a receptor(s) for PTH and/or ISO but not for AVP in the CNT and CT. although it remains to be clarified whether the reactivities to PTH and ISO in these cells originate from single or dual cells.
- Subjects
NEPHROLOGY; MONOCLONAL antibodies; KIDNEY tubules; RABBITS; CARBONIC anhydrase; ADENYLATE cyclase; PARATHYROID hormone; ISOPROTERENOL
- Publication
Kidney International, 1988, Vol 33, Issue 2, p543
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1988.32