We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Inulin prebiotic ameliorates type 1 diabetes dictating regulatory T cell homing via CCR4 to pancreatic islets and butyrogenic gut microbiota in murine model.
- Authors
Guimarães, Jhefferson Barbosa; Rodrigues, Vanessa Fernandes; Pereira, Ítalo Sousa; Manso, Gabriel Martins da Costa; Elias-Oliveira, Jefferson; Leite, Jefferson Antônio; Waldetario, Mariana Camila Gonçalves Miranda; Oliveira, Sarah de; Gomes, Arilson Bernardo dos Santos Pereira; Faria, Ana Maria Caetano; Ramos, Simone Gusmão; Bonato, Vânia L D; Silva, João Santana; Vinolo, Marco Aurélio Ramirez; Sampaio, Ulliana Marques; Clerici, Maria Teresa Pedrosa Silva; Carlos, Daniela
- Abstract
Gut dysbiosis is linked to type 1 diabetes mellitus (T1D). Inulin (INU), a prebiotic, modulates the gut microbiota, promoting beneficial bacteria that produce essential short-chain fatty acids for immune regulation. However, how INU affects T1D remains uncertain. Using a streptozotocin-induced (STZ) mouse model, we studied INU's protective effects. Remarkably, STZ + INU mice resisted T1D, with none developing the disease. They had lower blood glucose, reduced pancreatic inflammation, and normalized serum insulin compared with STZ + SD mice. STZ + INU mice also had enhanced mucus production, abundant Bifidobacterium , Clostridium cluster IV, Akkermansia muciniphila , and increased fecal butyrate. In cecal lymph nodes, we observed fewer CD4+Foxp3+ regulatory T cells expressing CCR4 and more Foxp3+CCR4+ cells in pancreatic islets, with higher CCL17 expression. This phenotype was absent in CCR4-deficient mice on INU. INU supplementation effectively protects against experimental T1D by recruiting CCR4+ regulatory T cells via CCL17 into the pancreas and altering the butyrate-producing microbiota.
- Subjects
REGULATORY T cells; TYPE 1 diabetes; GUT microbiome; ISLANDS of Langerhans; INULIN
- Publication
Journal of Leukocyte Biology, 2024, Vol 115, Issue 3, p483
- ISSN
0741-5400
- Publication type
Article
- DOI
10.1093/jleuko/qiad132