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- Title
IN VITRO REGENERATION OF BANANA GENOTYPES POSSESSING DISTINCT GENOMES BY USING MALE FLOWER EXPLANTS.
- Authors
LUSIYANTO; NURHASANAH; SUNARYO, W.
- Abstract
SLThe use of male flowers as explants offers an efficient protocol for overcoming the contamination problems encountered in plant propagation via tissue culture. This study aimed to evaluate the in vitro regeneration efficiency of commercial and local banana cultivars possessing different genomes by using male flower and flower base explants. Five commercial and local banana cultivars, i.e., 'Ambon' (AAA), 'Rutai' (AAB), 'Talas' (AAB), 'Kepok' (ABB), and 'Klutuk' (BB), were studied during 2020 at the Laboratory of Biotechnology, Department of Agroecotechnology, Mulawarman University, Samarinda, Indonesia. The nodule initiation stage was conducted in Murashige and Skoog medium containing indole acetic acid (IAA) (0.15, 0.30, and 1.00 mg L-1) combined with benzyl amino purine (BAP). After 8 weeks, the explants were transferred into multiplication media to induce shoot formation. The multiplication medium was the same as used at the initiation stage. However, the BAP concentration was increased 10-fold. Results revealed that in all the banana genotypes, the callus was dominantly induced at the initiation stage and failed to form shoots at the multiplication stage when male flower explants were used. However, by using male flower base explants, nodules and shoots were successfully induced in all genotypes with specific medium composition. High nodule formation was observed in the genotypes 'Talas' (on medium with 1 mg L-1 IAA combined with 2.00 or 4.00 mg L-1 BA) and 'Klutuk' (on medium with 0.30 mg L-1 IAA and 2.0 mg L-1 BAP). In these genotypes, 100% of the explants were able to form nodules. Nodules were successfully induced in the banana cultivars 'Talas' and 'Kepok' with 12-20, 19, and 2-5 shoots per explant at the multiplication stage. A high concentration of cytokinin (BAP) ranging from 20 mg L-1 to 40 mg L-1 in combination with low auxin (IAA) was found to be highly effective in inducing shoot formation in the genotypes 'Rutai', 'Talas', and 'Kepok'. The banana genotype 'Ambon' showed low shoot formation at the initiation stage. In conclusion, in vitro regeneration was successfully used and performed well in the local banana cultivars, 'Talas', 'Rutai', and 'Kepok' by using flower base explants.
- Subjects
CULTIVARS; BANANAS; GENOTYPES; INDOLEACETIC acid; GENOMES; FLOWERS; PLANT propagation
- Publication
SABRAO Journal of Breeding & Genetics, 2021, Vol 53, Issue 2, p322
- ISSN
1029-7073
- Publication type
Article