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- Title
Association of Interleukin-6 and Interleukin-1 Family Gene Polymorphisms in Autoimmune Hepatitis.
- Authors
Yousefi, Azizollah; Najafi, Mehri; Motamed, Farzaneh; Mahmoudi, Elham; Bidoki, Alireza Zare; Sadr, Maryam; Rahmani, Farzaneh; Farhmand, Fatemeh; Khodadad, Ahmad; Fallahi, Gholamhossein; Rezaei, Nima
- Abstract
Introduction and aim. Autoimmune hepatitis (AIH) is an immune-mediated destruction of liver cells, in recognition of interface hepatitis, seropositivity for autoantibodies, and interface hepatitis in histology sections. Hepatocyte destruction in AIH is the direct result of CD4+ T-cell destruction. Yet, Th17 mediated immune attach and a diversity of cytokine networks, including pro-inflammatory cytokines such as Interleukin 1 (IL-1) and Interleukin 6 (IL-6), set the stage for the destructive liver damage. Material and method. Peripheral blood samples from 57 patients, with AIH, recruited from referrals to the main pediatric hospital in Tehran. Single nucleotide polymorphisms for the following cytokines genes, were evaluated through, polymerase chain reaction with sequencespecific primers (PCR-SSP) assay: IL-1a (C/T -889), IL-1α (C/T -511), IL-1β (C/T +3962), IL-1 receptor (IL-1R; C/T Pst-I 1970), IL-1RA (C/T Mspa-I 11100), and IL-6 (C/G -174 and A/G nt565). Results. Significant higher frequency of genotype AA was detected in patients in IL-6 at position nt565 (15.8% in AIH patients vs. 2.9% in controls, p = 0.003). The haplotype GA of IL-6 at -174 and nt565, was significantly overrepresented in the AIH group, compared to (20.9% of AIH vs. 1.4% in controls p < 0.0001). Con- Conclusion. clusion. Results of our study, indicate significant deviation toward high yield IL-6 polymorphisms, in AIH patients. These data could bring new insights in pathophysiology of disease, which could contribute to developing novel treatments for AIH.
- Subjects
CHRONIC active hepatitis; GENETIC polymorphisms; INTERLEUKINS; AUTOANTIBODIES; LIVER cells; GENETICS of disease susceptibility
- Publication
Annals of Hepatology: Official Journal of the Mexican Association of Hepatology, 2018, Vol 17, Issue 6, p1021
- ISSN
1665-2681
- Publication type
Article
- DOI
10.5604/01.3001.0012.7202