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- Title
Mesangial factor V expression colocalized with fibrin deposition in IgA nephropathy.
- Authors
Liu, Ning; Ono, Takahiko; Suyama, Katsuo; Nogaki, Fumiaki; Shirakawa, Kiichi; Maeda, Mari; Kawamura, Takahide; Kamata, Tadashi; Oyama, Atsushi; Muso, Eri; Sasayama, Shigetake
- Abstract
Mesangial factor V expression colocalized with fibrin deposition in IgA nephropathy. Background. Factor V in its active form (Va) plays a key role at the termination of the intrinsic coagulation pathway, serving as a membrane-bound cofactor for the conversion of prothrombin to thrombin by factor Xa. Cross-linked fibrin (XFb) is often observed in mesangial areas in active types of human glomerulonephritis. In this study, to clarify contribution of factor V in intramesangial coagulation, mesangial factor V expression and its relationship to mesangial proliferation and fibrin deposition in IgA nephropathy (IgAN) were investigated. Methods. Twenty-two patients with IgAN were studied. XFb was detected in renal biopsy specimens using anti–d-dimer antibody combined with plasmin exposure, and factor V was detected with rabbit antibody against human factor V. Double-labeling immunohistochemistry was used to investigate the relationship of the glomerular distribution of factor V to XFb. The relationship of factor V staining to the activity index or XFb deposition was evaluated. The expression of factor V mRNA was assessed by in situ hybridization in relationship to the antigen staining of α-smooth muscle actin (α-SMA). The ultrastructural distribution of factor V in glomeruli was studied by immunoelectron microscopy. Results. XFb and factor V were observed in the mesangium and along capillary loops in seven and nine specimens, respectively. Factor V had intense, frequent expression in the proliferating and necrotizing areas, showing a significant relationship to XFb (P < 0.05). Furthermore, XFb deposition and factor V expression were markedly correlated with disease activity (P = 0.005 and P = 0.008, respectively). By double-labeling experiments, XFb and factor V were often seen colocalized in mesangial areas of the glomeruli, which showed necrotizing lesions and/or intense cellular proliferation. By in situ hybridization, factor V mRNA was detected...
- Subjects
KIDNEY diseases; FIBRIN
- Publication
Kidney International, 2000, Vol 58, Issue 2, p598
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1046/j.1523-1755.2000.00206.x