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- Title
The Inhibition Activity of Natural Methoxyflavonoid from Inula britannica on Soluble Epoxide Hydrolase and NO Production in RAW264.7 Cells.
- Authors
Kim, Jang Hoon; Han, Kyung-Sook; Lee, Eun-Song; Kim, Yong-Goo; Kim, Yong-Il; Cho, Byoung Ok; Lee, Ik Soo
- Abstract
Soluble epoxide hydrolase (sEH) is an enzyme targeted for the treatment of inflammation and cardiovascular diseases. Activated inflammatory cells produce nitric oxide (NO), which induces oxidative stress and exacerbates inflammation. We identify an inhibitor able to suppress sEH and thus NO production. Five flavonoids 1–5 isolated from Inula britannica flowers were evaluated for their abilities to inhibit sEH with IC50 values of 12.1 ± 0.1 to 62.8 ± 1.8 µM and for their effects on enzyme kinetics. A simulation study using computational chemistry was conducted as well. Furthermore, five inhibitors (1–5) were confirmed to suppress NO levels at 10 µM. The results showed that flavonoids 1–5 exhibited inhibitory activity in all tests, with compound 3 exhibiting the most significant efficacy. Thus, in the development of anti-inflammatory inhibitors, compound 3 is a promising natural candidate.
- Subjects
EPOXIDE hydrolase; COMPUTATIONAL chemistry; OXIDATIVE stress; FLAVONOIDS; NITRIC oxide; ENZYME kinetics
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 8, p4357
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25084357