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- Title
Hypothermia Inhibits Dexmedetomidine-Induced Contractions in Isolated Rat Aortae.
- Authors
Lee, Soohee; Hwang, Yeran; Park, Kyeong-Eon; Bae, Sungil; Ok, Seong-Ho; Ahn, Seung-Hyun; Sim, Gyujin; Bae, Moonju; Sohn, Ju-Tae
- Abstract
Dexmedetomidine is widely used to induce sedation in the perioperative period. This study examined the effect of hypothermia (33 and 25 °C) on dexmedetomidine-induced contraction in an endothelium-intact aorta with or without the nitric oxide synthase inhibitor NW-nitro-L-arginine methyl ester (L-NAME). In addition, the effect of hypothermia on the contraction induced by dexmedetomidine in an endothelium-denuded aorta with or without a calcium-free Krebs solution was examined. The effects of hypothermia on the protein kinase C (PKC), myosin light chain (MLC20) phosphorylation, and Rho-kinase membrane translocation induced by dexmedetomidine were examined. Hypothermia inhibited dexmedetomidine-induced contraction in the endothelium-intact aorta with L-NAME or endothelium-denuded aorta. Hypothermia had almost no effect on the dexmedetomidine-induced contraction in the endothelium-denuded aorta with the calcium-free Krebs solution; however, the subsequent contraction induced by the addition of calcium was inhibited by hypothermia. Conversely, the transition from profound hypothermia back to normothermia reversed the hypothermia-induced inhibition of subsequent calcium-induced contractions. Hypothermia inhibited any contraction induced by KCl, PDBu, and NaF, as well as PKC and MLC20 phosphorylation and Rho-kinase membrane translocation induced by dexmedetomidine. These results suggest that hypothermia inhibits dexmedetomidine-induced contraction, which is mediated mainly by the impediment of calcium influx and partially by the attenuation of pathways involving PKC and Rho-kinase activation.
- Subjects
PROTEIN kinase C; HYPOTHERMIA; NITRIC-oxide synthases; INDUCED hypothermia; AORTA; ENDOTHELIUM; METHYL formate
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 5, p3017
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25053017