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- Title
Pyrobayes: an improved base caller for SNP discovery in pyrosequences.
- Authors
Quinlan, Aaron R.; Stewart, Donald A.; Strömberg, Michael P.; Marth, Gábor T
- Abstract
Previously reported applications of the 454 Life Sciences pyrosequencing technology have relied on deep sequence coverage for accurate polymorphism discovery because of frequent insertion and deletion sequence errors. Here we report a new base calling program, Pyrobayes, for pyrosequencing reads. Pyrobayes permits accurate single-nucleotide polymorphism (SNP) calling in resequencing applications, even in shallow read coverage, primarily because it produces more confident base calls than the native base calling program.
- Subjects
GENETIC polymorphisms; LIFE sciences; SCIENTIFIC discoveries; NUCLEOTIDES; ERRORS
- Publication
Nature Methods, 2008, Vol 5, Issue 2, p179
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1172