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- Title
Impact of dengue virus (DENV) co-infection on clinical manifestations, disease severity and laboratory parameters.
- Authors
Dhanoa, Amreeta; Hassan, Sharifah Syed; Chin Fang Ngim; Chun Fatt Lau; Teik Seng Chan; Adnan, Nur Amelia Azreen; Wei Han Eng, Wilhelm; Han Ming Gan; Rajasekaram, Ganeswrie; Ngim, Chin Fang; Lau, Chun Fatt; Chan, Teik Seng; Eng, Wilhelm Wei Han; Gan, Han Ming
- Abstract
<bold>Background: </bold>The co-circulation of 4 DENV serotypes in geographically expanding area, has resulted in increasing occurrence of DENV co-infections. However, studies assessing the clinical impact of DENV co-infections have been scarce and have involved small number of patients. This study explores the impact of DENV co-infection on clinical manifestations and laboratory parameters.<bold>Methods: </bold>This retrospective study involved consecutive hospitalized patients with non-structural protein 1 (NS1) antigen positivity during an outbreak (Jan to April 2014). Multiplex RT-PCR was performed directly on NS1 positive serum samples to detect and determine the DENV serotypes. All PCR-positive serum samples were inoculated onto C6/36 cells. Multiplex PCR was repeated on the supernatant of the first blind passage of the serum-infected cells. Random samples of supernatant from the first passage of C6/36 infected cells were subjected to whole genome sequencing. Clinical and laboratory variables were compared between patients with and without DENV co-infections.<bold>Results: </bold>Of the 290 NS1 positive serum samples, 280 were PCR positive for DENV. Medical notes of 262 patients were available for analysis. All 4 DENV serotypes were identified. Of the 262 patients, forty patients (15.3 %) had DENV co-infections: DENV-1/DENV-2(85 %), DENV-1/DENV-3 (12.5 %) and DENV-2/DENV-3 (2.5 %). Another 222 patients (84.7 %) were infected with single DENV serotype (mono-infection), with DENV- 1 (76.6 %) and DENV- 2 (19.8 %) predominating. Secondary dengue infections occurred in 31.3 % patients. Whole genome sequences of random samples representing DENV-1 and DENV-2 showed heterogeneity amongst the DENVs. Multivariate analysis revealed that pleural effusion and the presence of warning signs were significantly higher in the co-infected group, both in the overall and subgroup analysis. Diarrhoea was negatively associated with co-infection. Additionally, DENV-2 co-infected patients had higher frequency of patients with severe thrombocytopenia (platelet count < 50,000/mm(3)), whereas DENV-2 mono-infections presented more commonly with myalgia. Elevated creatinine levels were more frequent amongst the co-infected patients in univariate analysis. Haemoconcentration and haemorrhagic manifestations were not higher amongst the co-infected patients. Serotypes associated with severe dengue were: DENV-1 (n = 9), DENV-2 (n = 1), DENV-3 (n = 1) in mono-infected patients and DENV-1/DENV-2 (n = 5) and DENV-1/DENV-3 (n = 1) amongst the co-infected patients.<bold>Conclusion: </bold>DENV co-infections are not uncommon in a hyperendemic region and co-infected patients are skewed towards more severe clinical manifestations compared to mono-infected patients.
- Subjects
DENGUE viruses; RETROSPECTIVE studies; SEVERITY of illness index; MIXED infections; VIRAL nonstructural proteins; REVERSE transcriptase polymerase chain reaction; NS1 viral protein; PLEURAL effusions; RNA metabolism; DIAGNOSIS; DENGUE; EPIDEMICS; BIOLOGICAL evolution; FLAVIVIRUSES; MULTIVARIATE analysis; POLYMERASE chain reaction; PROTEINS; RNA; SEROTYPES; ODDS ratio
- Publication
BMC Infectious Diseases, 2016, Vol 16, p1
- ISSN
1471-2334
- Publication type
journal article
- DOI
10.1186/s12879-016-1731-8