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- Title
Wide-bore P-methylstyrene-co-dimethylbis(p-vinylbenzyl) silane Based-monoliths Columns for Separation of Peptides and Proteins.
- Authors
Lubbad, Said H.
- Abstract
Wide-bore monolithic columns of p-methylstyrene-co-dimethylbis(p-vinylbenzyl)silane were prepared within glass columns (100 x 3 mm I.D.). Monolithic columns differing in the amount of initiator were fabricated. The pressure drop vs. flow-rate measurements and the relatively low swelling propensity factor indicated the good cross-linking homogeneity and good mechanically stability. The chromatographic performance of monoliths prepared was assessed by analysis of protein and peptide mixtures. Five proteins were separated in less than 2 minutes. Moreover, a mixture of 9 peptides was separated in less than 11 minutes, aside from the co-elution of vasopressin [arg8] and methionine enkephalin.
- Subjects
STYRENE; SILANE; SEPARATION (Technology); PRESSURE; ENKEPHALINS; CHROMATOGRAPHIC analysis; VASOPRESSIN; METHIONINE; MIXTURES
- Publication
Acta Chimica Slovenica, 2010, Vol 57, Issue 4, p880
- ISSN
1318-0207
- Publication type
Article