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- Title
A Fluorescence Immunochromatographic Strip Based on Quantum Dot Nanobeads for the Rapid Detection of Okadaic Acid.
- Authors
Wang, Han; Ren, Hong-Lin; Hu, Pan; Li, Yan-Song; Zheng, Yu; Cao, Qi; Liu, Zhan-Xu; Liu, Zeng-Shan; Yang, Yong; Lu, Shi-Ying
- Abstract
A fluorescence immunochromatographic strip using quantum dot nanobeads (QDNBs)-monoclonal antibody (McAb) conjugates was developed to rapidly detecting okadaic acid (OA) in shellfish. Under optimal conditions, the linear working range was 0.62–20 ng·mL−1, and the full competitive limit of detection (LOD) was 20 ng·mL−1, corresponding to 160 μg·kg−1 per shellfish, meeting the regulatory limit of European Conformity (EC). The strips could show results in 20 min, had high reproducibility, and were effective within 6 months when stored sealed at 4 °C and 25 °C. Moreover, the strips successfully detected OA in spiked shellfish samples. The reliability of the test strips was further confirmed by enzyme-linked immunosorbent assay (ELISA) which was consistent well with Abraxis imported ELISA (R2 = 0.988). The strips are sensitive, specific, replicable, and ideal for rapidly detecting OA, providing a potential tool that can be further developed to detect OA and other marine toxins in shellfish.
- Publication
Food Analytical Methods, 2022, Vol 15, Issue 9, p2470
- ISSN
1936-9751
- Publication type
Article
- DOI
10.1007/s12161-022-02302-6