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- Title
Orthodontic mechanical tension effects on the myofibroblast expression of alpha-smooth muscle actin.
- Authors
Yao Meng; Xianglong Han; Lan Huang; Ding Bai; Hongyou Yu; Yan He; Yan Jing
- Abstract
Objective: To detect myofibroblast formation on the tension side during orthodontic tooth movement in vivo and myofibroblast expression of alpha-smooth muscle actin (α-SMA) induced by tension both in vivo and in vitro. Materials and Methods: Fifty 6-week male rats were used in this in vivo study, and the right maxillary first molar was moved mesially, which served as the experimental group, and the left maxillary first molar served as the control. Rats were sacrificed at days 0, 3, 5, 7, and 14 after force loading. Myofibroblasts, identified with α-SMA, were examined through immunohistochemistry. For the in vitro study, human periodontal ligament (PDL) fibroblasts were obtained. Cyclic mechanical tension was applied to the fibroblasts for 0, 1, 3, 6, and 12 hours. Transmission electron microscopy was used to detect the ultrastructure of myofibroblasts. α-SMA mRNA gene expression was quantified by real-time quantitative PCR. The expression of α-SMA was detected by immunofluorescence and quantified by Western blotting. Results: In vivo, the myofibroblasts expressing α-SMA were identified both in the experimental group and in the control group. The expressions of α-SMA were increased in the tension areas of the experimental group over time, and reached the maximum in day 14. In vitro, fibronexus junctions and actin microfilaments in the cells could be found with transmission electron microscopy. Cyclic mechanical tension could significantly induce α-SMA expression at 12 hours (P < .01) than the controls. Conclusions: Myofibroblasts existed in the PDL. The expressions of α-SMA in the myofibroblasts were significantly up regulated under tension both in vivo and in vitro.
- Subjects
CORRECTIVE orthodontics; MYOFIBROBLASTS; LABORATORY rats; PERIODONTAL ligament; MESSENGER RNA
- Publication
Angle Orthodontist, 2010, Vol 80, Issue 5, p912
- ISSN
0003-3219
- Publication type
Article
- DOI
10.2319/101609-578.1