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- Title
IDENTIFICATION OF PANDAN PLANT (Benstonea sp) FROM RIAU, INDONESIA USING THREE DNA BARCODES.
- Authors
ROSLIM, DEWI INDRIYANI
- Abstract
Pandan from Riau is one of the important plants in Kajuik Lake located in Langgam, Riau Province of Indonesia, although its scientific name has not been recognized. This study reports the use of three DNA barcodes: matK, rbcL, and trnL-trnF intergenic spacer; to determine the Pandan's taxonomic status. The methods included DNA isolation, PCR, electrophoresis, and sequencing. The software BLASTn, BioEdit, and MEGA were used to analyze the data. The matK, rbcL, and trnL-trnF intergenic spacer sequences obtained were 639 bp, 539 bp, and 1014 bp in size, respectively. The results showed that although the identification had already been performed using two standard DNA barcodes sequences for plants, i.e. the matK and rbcL, and also the trnL-trnF intergenic spacer sequence which was commonly used as a DNA barcode in Pandanaceae and abundantly available in GenBank, none of them had 100% similarity to Pandan from Riau. In addition, the dendrogram generated from those sequences showed that Pandan from Riau had the closest relationship with a few species of Benstonea rather than Pandanus, Martellidendron, and Freycinetia. It can be concluded that the scientific name of Pandan from Riau can only be determined up to the genus level, i.e. Benstonea sp. This suggests that Pandan from Riau is an identified species but its DNA barcode sequences are not present in the GenBank database. It can also be understood that this plant is a new species of Benstonea which has never been identified and reported.
- Subjects
PANDANUS; PLANT genetic engineering; PLANT DNA; PLANT morphology; COMPLEMENTATION (Genetics)
- Publication
SABRAO Journal of Breeding & Genetics, 2017, Vol 49, Issue 4, p346
- ISSN
1029-7073
- Publication type
Article